A9043
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A9043
Sigma
S-Acetylthioglycolic acid N-hydroxysuccinimide ester
≥95% (TLC), powder
| Synonym: | N-Succinimidyl (acetylthio)acetate, N-Succinimidyl S-acetylthioglycolate, S-Acetylthioglycolic acid NHS ester |
| CAS Number: | 76931-93-6 |
| Linear Formula: | C8H9NO5S |
| Molecular Weight: | 231.23 |
| Beilstein Registry Number: | 7815491 |
| MDL number: | MFCD00036891 |
| PubChem Substance ID: | 24891221 |
Description
| Application | Thiolating reagent for primary amines; thiol can be deprotected under mild conditions. Typically, couples initially to a molecule containing primary amine by amide bond buffered at pH 7.5. Deprotection may be accomplished with 0.05 M hydroxylamine at neutral pH. Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates. |
| Other Notes | Note that the ester couples very efficiently to primary amines compared to other thiolating reagents. In addition, this reagent will neutralize original amine positive charge. |
Properties
| assay | ≥95% (TLC) |
| form | powder |
| solubility | acetonitrile: 50 mg/mL |
| DMF: soluble | |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| reference | Duncan, R.J., et al., A new reagent which may be used to introduce sulfhydryl groups into proteins, and its use in the preparation of conjugates for immunoassay. Anal. Biochem. 132, 68-73, (1983) Abstract |
